Automated Prediction of Lysine Modifications
on Proteins

Fast Analysis

Rapid processing of protein structures

Validated Algorithm

Experimentally validated predictions

Excel Export

Detailed results in spreadsheet format

Web-based

No software installation required

Web-based implementation of a tertiary-structure based approach that predicts lysine reactivity for protein modification

Quick Start Guide
File Requirements
  • PDB Structure: Standard PDB format with ATOM records
  • H-bond File: PDB with hydrogen atoms added
  • Charge File: PQR format with partial charges
Probe Radius Selection
  • 1.4 Å: Standard water accessibility
  • 4.2 Å: ATRP initiator size (recommended)
  • Custom: Match your specific initiator
Result Interpretation
  • FastHigh reactivity expected
  • SlowModerate reactivity
  • NonBuried or sterically hindered
Detailed File Preparation Guide
Automated File Preparation (Beta)
1
Upload Files
2
Processing
3
Results
File Upload

Drag and drop your PDB file here or click to browse

Main protein structure file (.pdb)

Drag and drop your H-bond file here or click to browse

PDB file for hydrogen bond analysis (.pdb)

Drag and drop your charge file here or click to browse

Charge information file (.pqr, .txt)
Å
Recommended values:
• 1.4 Å - Standard water probe (default)
• 4.2 Å - ATRP initiator probe (experimental validation)
• Custom - Based on your specific initiator size
Processing

Initializing...

Job ID:
Results